Liposome Encapsulation Service for microRNAs

MicroRNAs (miRNAs) is a small non-coding RNAs that play crucial roles in regulating various biological processes, including carcinogenesis. Recently, liposomes can precisely deliver miRNAs at high concentrations as an effective delivery tool. CD Formulation excels in utilizing liposome encapsulation technology to support clients in developing stable miRNA liposomal formulations.

Fig.1 The challenges of in vivo miRNA/siRNA delivery. (Kumar, Virender, et al, 2014)

Advantages of microRNA Liposome Encapsulation

• Targeting delivering. Liposomes can deliver drugs to specific cells or tissue targets.
• Prolonging the duration of the prescription. Successful delivery of miRNA expression vectors into cells is ensured without nuclease degradation.
• Protecting microRNA. Liposomes can effectively protect microRNA from inactivation or degradation.
• Non-immunogenic. Liposomes with cationic charge have a strong affinity for cell membranes with low immunogenicity.
• High cell membrane permeability. Liposomes can significantly improve the cellular uptake of microRNAs (miRNAs).

Categories of Liposome microRNA Encapsulation

At present, liposomes represent the most extensively utilized non-viral nanocarriers for microRNA delivery applications. The various types of liposomes can be primarily classified into the following categories:

• Cationic liposome. Liposomes with cationic charge have been widely used for delivering miRNA, but the main disadvantage is low efficiency. Therefore, various measures have been taken to address this challenge. For example, combining PEG with liposomes can promote their escape from phagocytosis, improving overall delivery efficiency.
• Neutral liposomes. Liposomes with neutral charge mainly can alleviate the adverse effects caused by cationic liposomes.
• Ligand-modified liposomes. In liposome formulations, we can achieve more targeted tissue delivery by using lipid ligands that can specifically bind to receptors on target cells. For example, aptamers can bind to cell surface receptors while antibodies can be specifically used to target cancer cells.

Fig.2 Strategies used for delivery and targeting of miRNA in vivo. (Kumar, Virender, et al, 2014)

Our Liposome-Based microRNA Encapsulation Service

Formulation of liposomes encapsulation for microRNAs

We aim to offer professional formulation services for microRNA-encapsulated liposomes. We specialize in the research of various micro RNAs and the formulation of tailored preparation strategies based on specific application scenarios.

Targeting services

We can formulate specialized targeting strategies specifically tailored for microRNA. There are various strategies we can employ to improve the targeted delivery of miRNA to specific cells or tissues, including PEG-modified cation technology, aptamer modification techniques, and antibody modification techniques.

Characterization service

To effectively deliver siRNA/lipid complexes to target cells, precise modulation of their size is essential to conform to the structural constraints of the target tissue. Following systemic administration, red blood cells, and endothelial cells readily interact with the siRNA/lipid complexes. We assist our clients in optimizing liposomal targeting towards specific cell types by regulating particle size.

Our Capacities for microRNA Liposome Encapsulation

Techniques and Platforms	Specifics
microRNA Encapsulation Technology	Liposome modification and targeting techniques. Liposome types and methods screening. Purification techniques. Size control techniques. Process research and parameter optimization.
Characterization Platform	In vitro evaluation platform (such as cell uptake rate test, cytotoxicity test, release rate, and stability). In vivo evaluation platform (including cell targeting test, in vivo stability, and distribution). Physical & chemistry evaluation platform (size distribution, potential analysis, and encapsulation efficiency).

Why Choose CD Formulation?

Superior Liposome Encapsulation Technology

• Targeted binding to specific cells and tissues.
• Advanced technology for the regulation of microRNA liposome particle size.
• The robust encapsulation platform is specifically designed for microRNA.

Exclusive Analytical Platform

• Advanced instruments and equipment.
• Proficient in formulating and implementing scientific analytical methodologies.
• A robust analytics platform designed to facilitate the transition from research to production.

Top Team

• Numerous interdisciplinary scientists
• Be proficient in the application of liposomes in medicine.
• Substantial expertise in the encapsulation of miRNA within liposomes.

Published Data

CD Formulation is committed to delivering innovative and efficient microRNA liposome encapsulation services, designed to meet the unique requirements of pharmaceutical companies and researchers. Our advanced solutions are tailored to support cutting-edge research and development projects in the field of nucleic acid therapeutics. Contact us to explore how we can contribute to your scientific endeavors.

How It Works

STEP 1

Submit a service request describing your specific research or development need.

STEP 2

We'll email you to provide your quote and confirm order details if applicable.

STEP 3

Execute the project with real-time communication, and deliver the final report promptly.