Liposome Encapsulation Services

In nanoparticle (NP)-mediated drug delivery, liposomes are the most widely utilized drug carriers and represent the only NP system approved by the FDA for clinical use, owing to their superior physical and chemical properties and excellent biocompatibility. Currently, liposome technology enables the production of safe and effective systems on an industrial scale. CD Formulation possesses extensive expertise and many years of experience in drug delivery and liposome biophysics, demonstrating our commitment to leveraging professional knowledge to provide comprehensive solutions for our clients.

About Liposome Encapsulation Technology

Liposomes have a wide range of applications, spanning the fields of medicine, cosmetics, health products, and in vivo imaging. As an effective means, they can deliver targeted payloads (such as small molecules, DNA, and peptides) to specific cells or tissues within the body, regardless of whether the payloads are hydrophobic or hydrophilic. Here is an elaborate elucidation on the encapsulation of liposomes and its underlying operational principle.

Liposome Structure

• Liposomes are typically composed of a lipid bilayer, which consists of two layers of phospholipid molecules. These molecules possess a hydrophilic head and a hydrophobic tail.
• In aqueous environments, lipids naturally self-assemble into spherical vesicles, with hydrophobic tails oriented towards the interior and hydrophilic heads facing the exterior, thereby creating a water-containing cavity within the vesicle.

Liposome Encapsulation Principles

• To encapsulate substances in liposomes, the desired substance is combined with lipids and a water solution. Subsequently, the mixture undergoes mechanical or chemical treatments, such as ultrasonic treatment or extrusion. These processes result in the formation of liposomes, wherein the encapsulated substance is entrapped either within a water core or a lipid bilayer, depending on the properties of the substance and lipids.
• Conventional encapsulation techniques involve passive or active methods to load the payload into a hydrophilic core or a hydrophobic lipid bilayer based on its properties. In active loading, the payload can be incorporated into the liposome core post-synthesis by utilizing pH or salt gradients, and it is retained in the core after gradient-induced endocytosis through complex formation with the capture agent (ion/salt).
• The payload can be incorporated into the liposome through chemical bonding. For example, a payload with suitable functional groups can be conjugated to the phospholipid bilayer of the liposome using diverse chemical techniques. Common chemical bonds include disulfide, ester, and imine bonds, which release the payload under specific conditions. This approach enables the incorporation of the payload during synthesis (within the core and on the surface) or post-synthesis (only outer surface attachment).

Fig.1 Various modes of liposome encapsulation. (Almeida B, et al., 2020)

Customized Liposome Encapsulation Solutions

Liposome Encapsulation Service for Small Molecules

Small molecule drugs exhibit reduced selectivity and specificity, and undergo rapid metabolism and clearance by the body, resulting in a shorter half-life. Liposomes can protect encapsulated small molecule substances from degradation, dilution, and early inactivation in circulation.

Liposome Encapsulation Service for mRNAs

To address the challenges of mRNA, including instability, susceptibility to degradation, cellular uptake barriers, and strong immunogenicity.
Carriers employed for mRNA delivery include liposomes (LPs), lipid nanoparticles (LLPs), solid lipid nanoparticles (SLNs)...

Liposome Encapsulation Service for siRNAs

Lipid nanoparticles (LNPs) serve as the primary vehicle for in vivo delivery of small interfering RNA (siRNA) in therapeutic applications.
LNP siRNA system has demonstrated the ability to intravenously silence treatment-related genes across a range of...

Liposome Encapsulation Service for microRNAs

Liposomes protect cargo molecules such as RNA and DNA from degradation or inactivation.
Liposomes enhance the transport of cargo molecules to cells.
Liposomes loaded with miRNA could destroy cancer cells without affecting healthy tissue...

Liposome Encapsulation Service for Plasmid DNA

Liposomes shield DNA from enzymatic degradation within cells and also safeguard the encapsulated contents from degradation.
To improve the delivery of encapsulated DNA to diverse cell types, including plant cells. Furthermore, they can...

Liposome Encapsulation Service for Peptides

To shield peptides from the acidic conditions of the stomach and other harsh environments in the intestines, thereby facilitating their passage into the bloodstream and improving absorption and uptake rates.
Encapsulation can aid...

Liposome Vaccine Encapsulation Service

Compared to other vaccine adjuvants, liposomes offer numerous unique advantages, including (1) biocompatibility, biodegradability, low toxicity, and antigenicity; (2) sustained and controlled release; (3) adjustable targeting; and (4) inhibition of nucleic...

Our Capabilities for Liposome Encapsulation

Why Choose CD Formulation for Liposome Encapsulation?

• Advanced Liposome Technology: Our cutting-edge liposome encapsulation technique excels in research on drug delivery, gene therapy, cell engineering, and regenerative medicine. We precisely control liposome structure and composition for efficient payload delivery, ensuring stability, bioavailability, and targeted delivery within the body.
• Comprehensive Encapsulation Services: We offer professional liposome encapsulation services, providing innovative solutions for stabilizing and enhancing the bioavailability of active ingredients. Our expertise spans pharmaceuticals, cosmetics, and food supplements. Our services cover all aspects of the process, encompassing custom formula development, scaled-up production, and quality testing.
• Professional Team: The interdisciplinary research team, comprising experts in fields including basic medicine, molecular biology, chemistry, and pharmacy, collaborates to drive innovation and application of liposome encapsulation technology. They also integrate artificial intelligence technology to achieve breakthroughs in big data analysis, simulation calculation, and intelligent-assisted design, enhancing the team's innovative capabilities and problem-solving flexibility.

As a leader in nanoparticles, CD Formulation is committed to delivering professional liposome encapsulation services and superior liposome products to our customers. Please do not hesitate to contact us if you require any assistance.

How It Works

STEP 1

Submit a service request describing your specific research or development need.

STEP 2

We'll email you to provide your quote and confirm order details if applicable.

STEP 3

Execute the project with real-time communication, and deliver the final report promptly.