RNA-Conjugated Peptide Synthesis

RNA-conjugated peptides are useful pharmaceuticals because they have higher stability than RNA. CD Formulation provides custom synthesis and conjugation of peptide and RNA oligomers. RNA is conjugated to peptides via functionalized phosphoramidite monomers, the most common reactive moieties include amines, thiols, alkoxyamines or hydrazines, aldehydes, azides/alkynes. We also provide RNA-conjugated peptide modification services.

What Are RNA-Conjugated Peptides?

RNA-conjugated peptides represent a unique fusion of RNA and peptides. RNA and peptide conjugates have been used in a variety of therapeutic applications, such as RNA therapeutics, drug delivery, and screening peptide libraries in mRNA display approaches. The peptide portion can be used as a targeting ligand, specifically binding to certain receptors or cells. The RNA component can then be used to deliver therapeutic molecules or information, such as gene silencing.

Fig. 1 Formation or synthesis of peptido RNA. (Maximilian, et al., 2020)

Explore Our Custom RNA-Conjugated Peptide Synthesis Services

CD Formulation offers custom synthesis options for several peptides and RNAs listed below, but we are happy to discuss conjugation projects using your own RNA or peptide.

• Double-stranded RNA-peptide conjugates.
• RNA-peptide-RNA conjugates.
• Conjugation of single amino acids to RNA.
• Labeling peptide-RNA complexes with fluorescent dyes or other modifications.

Our methods for preparing peptide-RNA conjugates include:

• Post-synthesis conjugation (or post-assembly conjugation and fragment conjugation strategies).
• Fully stepwise synthesis (or online solid phase synthesis).
• Native and template-directed ligation.

Our DNA-Peptide Conjugation Strategies

Fig.2 Schematic diagram of RNA and peptide conjugation. (CD Formulation)

NHS Ester-maleimide Mediated Conjugation Chemistry

Our scientists use NHS ester-maleimide mediated conjugation chemistry to synthesize RNA-peptide conjugates. Similar to DNA-peptide synthesis, this strategy involves reacting N/C-terminal Cys-conjugated peptides with maleimide-activated RNA. If the peptide has an internal Cys, we will use other strategies such as reacting hydroxylamine-modified peptides with aldehyde-modified oligonucleotides to form oximes.

RNA-Conjugated Peptide Modification

Modifications can be incorporated into the RNA or peptide molecules, or both molecules (if appropriate). Modifications include:

• Phosphorothioate bonds on the backbone of the RNA molecule.
• Modified bases on the RNA.
• Modified amino acids incorporated into the peptide sequence.
• Fluorescent and dye labels, including but not limited to FAM, TAMRA, BHQ1, and BHQ2.
• Biotinylation
• Spacers

Delivery Specifications of RNA-Conjugated Peptide

Peptide Manufacturing & Analytical Services

In addition to peptide synthesis capabilities, CD Formulation combines flexible GMP manufacturing facilities with cutting-edge peptide analytical knowledge to provide a full range of quality control testing services to accelerate the commercialization of your products, including:

Publication

CD Formulation is a trusted partner for peptide synthesis. Please don't hesitate to contact us if you are considering using RNA-conjugated peptides in your project. We look forward to cooperating with you.

How It Works

STEP 1

Submit a service request describing your specific research or development need.

STEP 2

We'll email you to provide your quote and confirm order details if applicable.

STEP 3

Execute the project with real-time communication, and deliver the final report promptly.