α-Hydroxy Acid Test

α-Hydroxy acids are also known as hydroxy acids. Hydroxy acids are a general term for organic acids extracted from fruits. These organic acids have in common the presence of a hydroxyl or keto group in the alpha position of the molecular structure, and are often referred to as alpha-hydroxy or keto acids. These organic acids are found naturally in substances such as fruit, sugar and yoghurt.

CD Formulation can provide α-hydroxy acid detection services to help customers quickly understand their product performance.

alfa-Hydroxy Acid Test

About the α-Hydroxy Acid of Cosmetics

α-Hydroxy acid is a class of small molecules, easy to be absorbed by the skin, with moisturizing, anti-wrinkle, whitening and other effects, is widely used in cosmetics, but the use of α-hydroxy acid will have irritating effects, and even lead to skin redness, blistering and burns. Therefore, it is of great significance to establish an accurate and reliable method for the detection of α-hydroxy acid in cosmetics.

Commonly used α-hydroxy acids
No. Name
#1 Glucuronic acid
#2 Tartaric acid
#3 Glycolic acid
#4 Malic acid
#5 Lactic acid
#6 Citric acid
#7 DL-2-Hydroxybutyric Acid Sodium
#8 Mandelic acid
#9 Benzilic acid
#10 Hydroxycaprylic acid

α-Hydroxy Acid Test

CD Formulation offer three detection methods, including high performance liquid chromatography, gas chromatography and ion chromatography. These three methods are suitable for the determination of α-hydroxy acids in cosmetics for washing, hair care and skin. The detailed procedure is as follows.

1) Standard reserve solution for preparing α-hydroxyl acid and dilute into solutions of different concentrations to obtain standard series solutions;

2) Weighing samples, make sample solutions after a certain front processing;

3) Use high -efficiency liquid chromatography, gas chromatography or ion chromatography to detect standard series solutions and sample solutions;

4) Draw the standard curve of the standard series solution of α-hydroxyl acid,

If analysis of high-efficiency liquid chromatographs, it is qualitative to retain the time and ultraviolet spectrum chart, and the peak area is quantitative;

If the ion chromatography is used for analysis, the time is qualitative and the peak area is quantitative;

If the gas color spectrometer is analyzed, it is qualitative to retain the time and the peak area is quantitative.

Inspeal the α-hydroxyl acid in cosmetics for qualitative and quantitative testing.


  • During analysis, all solutions should be made to clarify to avoid blocking the instrument.

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