Microbial Contamination Test

Microbial testing of cosmetics and personal care products is an important quality control process to ensure product safety throughout the supply chain. CD Formulation performs a series of microbial tests to help you evaluate the safety or efficacy of raw materials, ingredients, raw materials and final products to verify the risk of product contamination under normal conditions of use.

Microbiological Testing Techniques for Cosmetics

Rapid Microbiological Testing Technique

The procedure of this method is: membrane filtration of microcolonies, followed by fluorometric observation of colonies. The specific method is that certain cosmetic samples are membrane filtered, and then the membrane filtered is placed on the liquid medium for a period of moderate temperature incubation, and finally the membrane is placed on top of a paper sheet dipped in ANS solution, then dried, and finally observed by fluorescence microscopy.

Rapid Detection Technique

This technique usually takes 6-12 hours to perform and is commonly used for radioactive tracing and impedance testing. Impedance test method, its test when the number of colonies below 107 / mL, it will lead to no change in resistance, and when the cosmetics sample contains bacteria the higher the period of detectable changes in resistance will be easier to determine.

Techniques for Calculating the Total Number of Microorganisms in Cosmetics

The total number of colonies is generally determined by averaging the values of two dishes.

Microbial Contamination Test

Microbial test assesses microbial indicators including: total bacteria, mold and yeast, Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, Salmonella, Candida albicans, Clostridium perfringens, Enterobacter spp. and Gram-negative bacteria and bile-tolerant Gram-negative bacteria, etc.

Service content

• Aerobic colony count

This test determines the total number of aerobic bacteria present per milliliter or gram of personal care or cosmetic product.

• Yeast and mold count

This test determines the total number of yeasts and fungi present per milliliter or gram of personal care or cosmetic product.

• Enrichment method for specific pathogen screening

This test determines if no harmful bacteria are present - even in very small numbers or quantities.

• Total gram negative test

This test looks for Gram-negative organisms, including

Pseudomonas aeruginosa, Escherichia coli, Salmonella, Serratia, Enterobacter, Klebsiella pneumoniae, etc.

Handling Precautions

Distilled water is most suitable for the preparation of culture medium, because distilled water has fewer impurities, and the medium made is highly transparent, which is conducive to the observation of microbial culture form and changes in life activities. The medium generally contains various nutrients required by microorganisms, such as carbon source, ammonia source, inorganic salts, growth factors and water. In order to avoid nutrient destruction of the medium, the sterilization temperature and time should be strictly controlled during the sterilization process, and the sterilization temperature or sterilization time should not be increased arbitrarily. In order to check and control the sterilization effect, each microbiological testing operation should carry out blank control work to check whether the used items have been completely sterilized, and to verify whether the operation process is in line with the aseptic operation.

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