Preservative Content Test

Preservatives can inhibit the growth of microorganisms and ensure the safety and effectiveness of cosmetics during production, use and preservation. CD Formulation can provide preservative content testing services to help customers quickly understand sample performance and better conduct follow-up research.

About the Preservative of Cosmetics

In the process of production and use of cosmetics, it is inevitable that there will be microbial invasion. If you do not add effective ingredients to inhibit microorganisms, it may deteriorate, not reach the desired effect, and even threaten the health of the user. Preservative is a substance added to cosmetics for the purpose of inhibiting microorganisms in cosmetics.

Preservative Content Test

At present, there are high performance liquid chromatography, gas chromatography and gas chromatography-mass spectrometry for the determination of preservatives in cosmetics. High performance liquid chromatography (HPLC) is the most widely used preservative analysis method at present. Because most of the preservatives are macromolecular organic compounds, and have UV absorption characteristics, it is more suitable for HPLC analysis. This method has the advantages of simple operation and high sensitivity.

CD Formulation provides these detectors, which can test the sample efficiently and quickly, helping customers get the content of preservatives in cosmetics. The detailed procedure is as follows.

  1. Prepare the standard solution of various preservatives respectively, and then dilute it with diluent into 6 solutions of different concentrations to obtain the mixed standard solution;
  2. Weigh the sample, add diluent to dissolve and dilute the sample solution;
  3. Liquid chromatography is used to detect the mixed standard solution and sample solution respectively;
  4. Draw the standard curve of preservative standard mixed solution series, and conduct qualitative and quantitative detection of preservatives in cosmetics by comparing the retention time and standard curve.

Preservative Content Test

Permissible preservatives for cosmetics
No. Name of preservatives No. Name of preservatives
1 Bronopol (INN) 25 1,6-Di(4-amidinophenoxy)-n-hexane and its salts (including isethionate and p-hydroxybenzoate)
2 5-Bromo-5-nitro-1,3-dioxane 26 Hexetidine (INN)
3 5-Ethyl-3,7-dioxa-1- azabicyclo[3.3.0]octane 27 3,3'-Bis(1-hydroxymethyl-2,5- dioxoimidazolidin-4-yl)-1,1'- methylenediurea
4 Alkyl(C12-C22) trimethyl ammonium bromide and chloride 28 Inorganic sulfites and hydrogensulfites
5 Benzalkonium chloride, bromide and saccharinate 29 3-Iodo-2-propynylbutylcarbamate
6 Benzethonium chloride 30 2-Methylisothiazol-3(2H)-one
7 Benzoic acid, its salts and esters 31 Mixture of 5-chloro-2- methylisothiazol-3(2H) -one and 2- methylisothiazol-3(2H)-one with magnesium chloride and magnesium nitrate (of a mixture in the ratio 3:1 of 5-chloro- 2- methylisothiazol 3(2H)-one and 2-methylisothiazol-3 (2H)-one)
8 Benzyl alcohol 32 4-Isopropyl-m-cresol
9 Benzylhemiformal 33 Biphenyl-2-ol and its salts
10 6,6-Dibromo-4,4-dichloro-2,2'- methylene-diphenol 34 4-Hydroxybenzoic acid and its salts and esters
11 Chlorhexidine (INN) and its digluconate,diacetate and dihydrochloride 35 4-Chloro-m-cresol
12 Chlorobutanol (INN) 36 2-Phenoxyethanol
13 4-Chloro-3,5-xylenol 37 1-Phenoxypropan-2-ol
14 3-(p-chlorophenoxy)-propane-1,2- diol 38 1-Hydroxy-4-methyl-6(2,4,4- trimethylpentyl)2-pyridon and its monoethanolamine salt
15 1-(4-chlorophenoxy)-1-(imidazol-1- yl)-3,3-dimethylbutan-2-one 39 Poly(methylene),.alpha.,.omega.-bis[[[(aminoiminomethyl)amino]iminomethyl]amino]-, dihydrochloride
16 3-Acetyl-6-methylpyran-2,4 (3H)- dione and its salts 40 Propionic acid and its salts
17 N-(Hydroxymethyl)-N-(dihydroxymethyl-1,3-dioxo-2,5-imidazolinidyl-4)-N'- (hydroxymethyl) urea 41 Salicylic acid and its salts
18 3,3'-Dibromo-4,4'- hexamethylenedioxydibenzamidine and its salts (including isethionate) 42 Silver chloride deposited on titanium dioxide
19 2,4-Dichlorobenzyl alcohol 43 Sodium hydroxymethylamino acetate
20 4,4-Dimethyl-1,3-oxazolidine 44 Sorbic acid (hexa-2,4-dienoic acid) and its salts
21 1,3-Bis(hydroxymethyl)-5,5- dimethylimidazolidine-2,4-dione 45 Triclocarban (INN)
22 Ethyl-N-alpha- dodecanoyl-Larginate hydrochloride 46 Triclosan (INN)
23 Formic acid and its sodium salt 47 Undec-10-enoic acid and its salts
24 Glutaraldehyde (Pentane-1,5-dial) 48 Pyrithione zinc (INN)


  • During liquid-phase analysis, all solutions should be made to clarify to avoid blocking the instrument.
  • Most preservatives have certain toxicity to human body. Different preservatives have different levels of toxicity, so the amount of use should be determined according to their toxicity intensity.

If you have a requirement about our services, please contact us by phone or email, our colleagues will reply to you within three working days.

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Please note: Our products and services are not intended to be used directly in diagnostic or therapeutic procedures.
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