Sex Hormones Test

Sex hormone is a kind of steroid hormone, including estrogen, androgen and progesterone. It has the function of controlling sexual organs and secondary sexual characteristics, and at the same time, it is involved in the metabolic activities of basic life substances such as sugar, fat, protein and inorganic salt in the human body, which is an important influence on reproductive health. Therefore, when sex hormones directly act on the skin, in the short term, it can promote hair growth, prevent skin aging, remove skin wrinkles, increase skin elasticity, and promote breast development. However, long-term use will lead to pigmentation, dark spots, skin atrophy and thinning, abnormal development and human metabolic disorders and other side effects, and even carcinogenic.

Sex Hormones Test

How to Test Sex Hormones

Due to the harm of sex hormones added in cosmetics to the human body, research on the detection methods of sex hormones in cosmetics has also attracted much attention in recent years. Commonly used methods for testing sex hormone components in cosmetics include high performance liquid chromatography, ultra-high performance liquid chromatography, liquid chromatography-mass spectrometry and gel permeation chromatography-liquid chromatography -tandem mass spectrometry.

Sex hormones involved in inspection standards
No. Sex hormones No. Sex hormones
1 Estriol 2 Norgestrel
3 Estrone 4 Megestrol acetate
5 Diethylstilbestrol 6 Chlormadinone acetate
7 Estradial 8 Medroxyprogesterone 17-acetate
9 Testosterone 10 Hydroxyprogesterone caproate
11 Methyltestosterone 12 Norethisterone
13 Progesterone 14 Melengestrol acetate
15 17α-hydroxyprogesterone acetate 16 Levonorgestrel
17 Mifepristone 18 Dydrogesterone
19 Gestrinone
  • High performance liquid chromatography

High performance liquid chromatography is a common method for the determination of sex hormones in cosmetics. It not only has a wide measurement range, high separation efficiency, fast analysis speed, but also requires a small amount of sample, and can be equipped with different detectors. It has the most commonly used detection method. HPLC can detect sex hormones in various cosmetics.

  • Ultra-high performance liquid chromatography

Compared with high performance liquid chromatography, ultra-high performance liquid chromatography technology can greatly improve the analysis speed, resolution, sample throughput and sensitivity of liquid chromatography, which can make the operation simple, fast and easy to popularize.

  • Liquid chromatography-mass spectrometry

Compared with liquid chromatography, liquid chromatography-mass spectrometry does not require complete separation of chromatographic peaks, which can overcome the shortcomings of complex pretreatment of other methods, thereby shortening sample analysis time, and has good specificity and higher sensitivity, especially for cosmetics. Qualitative and quantitative analysis of banned components in cosmetics, therefore, the detection method for the determination of various sex hormones in cosmetics by liquid chromatography-mass spectrometry has been widely used.

Sex Hormones Test

Our Services

CD Formulation, as a professional one-stop solution for cosmetics provider, offers a series of acosmetics one-stop testing services, and provides common testing equipment, which can test the sample efficiently and quickly, helping customers get the content of sex hormones in cosmetics.

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  1. Cheng Jun, et al. Simultaneous determination of eight sexual hormones in cosmetics by high performance liquid chromatography with diode array and fluorescence detectors. Chinese Journal of Analysis Laboratory. 2016, 35(1): 112-116.
  2. Zhang Qiuyan, Luo Huitai. Research progress on analytical techniques for hormone illegally added in cosmetics. Guangdong Chemical Industry, 2018, 45(374): 155-156.
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