Colorant Test

The main function of adding pigments in cosmetics is coloring, which not only has the functions of beautifying, modifying, and covering up, but also can adjust the influence of certain raw materials on the color of the product. Most of the colorants used in cosmetics are synthetic dyes, and long-term or excessive use will cause potentially harmful to human health, some colorants can cause allergic reactions, some can cause inflammation of eyes, mouth and other organs, and some can be absorbed by the human body through the skin, which has obvious mutagenic effects. Long-term use of such products can even induce cancer. At present, cosmetics have become the necessities of life for some people. For the sake of safety, many countries in the world have made relevant regulations on colorants used in cosmetics. Therefore, the detection of colorants in cosmetics has attracted more and more attention from consumers.

Colorant Test


According to the "Safety and Technical Specifications for Cosmetics", there are as many as 157 types of cosmetic colorants available, and different cosmetic colorants have different application scopes, prohibited products, maximum detection limits, and concentration. Therefore, the detection of colorants for cosmetics can better help us understand the types of colorants used in cosmetics, whether they meet the detection limit requirements, and whether they are suitable for the research and development requirements of cosmetic formulations.

Test Method for Colorant in Cosmetics

The detection methods of colorants for cosmetics mainly include liquid chromatography -ultraviolet detector method, liquid chromatography-diode detector method, liquid chromatography-mass spectrometry, thin layer chromatography, gas chromatography, gas chromatography-mass spectrometry, etc. The pretreatment techniques mainly include microwave extraction method, ultrasonic advance method, liquid-liquid extraction method and so on. The main method for detecting colorants in cosmetics is liquid chromatography. Pretreatment techniques include comparative dialysis, polyamide powder adsorption, and SPE solid-ultraviolet detection and extraction column method. Among them, the polyamide powder adsorption method has the highest recovery rate and accuracy, and is most suitable for the quantitative determination of synthetic colorants in cosmetics.

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There are usually 7 groups of colorants in hair dye and perm cosmetics, namely Basic Orange 31, Basic Yellow 87, Basic Red 51, Basic Violet 14 (CI42510), Acid Orange 3 (CI10385), Acid Violet 43 (CI60730), basic blue 26 (CI44045), in addition, there are about 10 groups of colorants used in cosmetic cosmetics such as rouge, lipstick, foundation, nail polish, mascara, eye shadow, respectively CI59040, CI16185, CI16255, CI10316, CI15985, CI16035, CI14700, Orange I, CI45380, CI15510. After our expert team extracts the samples, they are separated by high-performance liquid chromatography and detected by a diode array detector. According to the retention time and ultraviolet spectrum, the peak area is quantified, and its content is calculated by the standard curve method.

Why to Choose CD Formulation?

CD Formulation has more than ten years of experience in cosmetic testing, and has mastered a large number of cosmetic colorants and dyes testing technologies, especially high-throughput, ultra-trace detection technology research, which can accurately evaluate the qualitative and quantitative evaluation of cosmetic colorants. In addition, our expert team can carry out method specificity, linearity, detection limit, precision, recovery rate, stability and other methods according to the interference problems of different colorant sample pretreatment conditions, different sample matrices, and different quantitative methods. The scientific verification test is carried out to test the actual colorant samples for cosmetics.

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If you have a requirement about our services, please contact us by phone or email, our colleagues will reply to you within three working days.

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Please note: Our products and services are not intended to be used directly in diagnostic or therapeutic procedures.
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