Excessive stress on the skin can lead to damage to the skin barrier, which can lead to skin symptoms such as burning, dryness, flaking, tingling, tightness, etc., and induce a large number of inflammatory factors to be expressed and generated. CD Formulation can carry out a soothing efficacy evaluation to assess the safety, efficacy of the ingredients and other indicators, and issue a professional soothing efficacy evaluation report.
The soothing efficacy of cosmetics mainly focuses on the burning, stinging, itching, tightness, erythema, scaling, redness (capillary dilatation) and other symptoms of the skin caused by damage to the skin barrier, abnormal nerve function, and inflammation, etc., and quantitatively analyzes the relevant active ingredients of cosmetics through physical and chemical analytical methods such as skin stratum corneum sampling, liquid chromatography, ionization spray mass spectrometry, tandem mass spectrometry, and so on.
Biochemical method refers to a method to evaluate the efficacy of cosmetics by testing and analyzing the characteristic indexes and important biomolecule contents that affect the efficacy of cosmetics with the help of spectral analysis, electron microscope, and other physical and chemical technical means through the biochemical reactions occurring in vitro, such as tyrosinase inhibition test, hyaluronidase inhibition test, and in vitro antioxidant test.
Cell biology method evaluates the efficacy of cosmetics by simulating the human physiological environment, cultivating cells in vitro and establishing cell models, and detecting the changes in relevant indicators before and after the cells are treated by the test substance.
The instrumental detection indexes of human test include skin water loss rate, skin erythema index, skin moisture content, skin surface pH, etc.
3D skin model refers to the artificial skin tissue model with three-dimensional structure constructed by using tissue engineering technology to culture human skin cells on a special inserted petri dish, which can replace the animal model to a certain extent.
In the soothing assessment system, the main model used is the skin damage model induced by low concentration of SLS, which causes localized skin irritation, and it leads to changes in certain proteins of epidermal differentiation, such as filamentous proteins, keratins, etc., which are very critical for the skin barrier, and these proteins are down-regulated in response to the stimulation of SLS. This damage modeling system can be used to screen for formulations that can alleviate the inflammation of skin irritation triggered by chemical-based reagents, surface activation, or excessive physical damage, as well as for formulations that can repair tissues and enhance the levels of barrier-related proteins.
Sunburn is characterized by redness and peeling of the skin, with the main contributing factor being UV, which leads to the accumulation of large amounts of reactive oxygen species (ROS) in the skin, which in turn leads to DNA damage in the skin cells and inflammation of the skin. We have established a soothing efficacy evaluation system for sunburn - by quantifying the number of sunburned cells in sunburned skin and the characteristic products of DNA damage in the cell nucleus, we can evaluate whether the product is able to alleviate sunburn-induced cell damage, apoptosis, and whether it can carry out repair after DNA damage.
There are many people in the hot and cold alternating environment, will feel the skin discomfort, such as skin dryness, tightness, burning, etc., these are related to nerve sensitivity, in the epidermis of the keratinized cell membrane, there are a variety of sensory receptors, a large proportion of which are ionic channels, when subjected to external pressure and stimulation, there will be calcium ion inward flow phenomenon occurs. Clinically, sensitive skin often experiences burning, stinging, and itching, all of which are closely associated with transient receptor potential channels (TRPV).