Antimicrobial Effectiveness Testing

Antibacterial is defined as can kill microorganisms, or within a specific time keeping microbial growth or reproduction under normal level, these microbes are usually bacteria or mold, when growth may produce odor, or generate biofilms slimy feeling or dirt spots, so products antibacterial appeal is after using the product, can be killed by bacteria or inhibit bacteria growth, achieve The effect of cleaning and deodorizing. Antimicrobial effectiveness testing is conducted in accordance with USP <51> antimicrobial potency testing, EP 5.1.3 antimicrobial preservative potency, and CTFA preservative challenge testing. The following microorganisms are required to be tested under USP <51>: escherichia coli, staphylococcus aureus, candida albicans, aspergillus brasiliensis, and pseudomonas aeruginosa.

Our Services

CD Formulation can perform antimicrobial effectiveness testing (AET) or preservative effectiveness testing to help evaluate your product's ability to resist microbial contamination during use. Our experts provide this testing for injectables packaged in multi-dose containers and for products containing antimicrobial preservatives. We also test products in water-based, multi-dose topical and oral dosage forms, as well as drug dosage forms including ear, eye, rinse, nasal and dialysis solutions.

We perform antimicrobial effectiveness testing for method suitability and routine testing. Our bioburden testing capabilities include:

Membrane filtration method: a quantitative technique that uses membrane filters and vacuum to isolate bacterial and fungal colonies from fluid samples.

Standard Plate Counting / Inverted Plate Method: Isolation of bacteria and fungi by spreading sample preparations onto Petri dishes and adding nutrient agar that allows the growth of any microorganisms present.

Diffusion plate method: isolation of bacteria and fungi by spreading sample preparations onto nutrient agar plates.

Service Flow for Antimicrobial Effectiveness Testing

  • Contact us by email to inform our staff of your request.
  • Sign the relevant confidentiality agreement to ensure mutual benefit.
  • Develop an appropriate experimental plan for your product needs.
  • Perform tests and ensure data reliability.
  • Send the data and analysis results to the customer.

Service Flow of CD Formulation for Antimicrobial Effectiveness Testing

How to Contact Us?

If you have a requirement about our services, please contact us by phone or email, our colleagues will reply to you within three working days.


  1. Jia, M.; et al. Antibacterial effect of S-Porphin sodium photodynamic therapy on Staphylococcus aureus and multiple drug resistance Staphylococcus aureus[J]. Photodiagnosis and Photodynamic Therapy, 2019, 28.
  2. Xiao-Qiong, G. U.; et al. Antibacterial effect anlysis of polygonum cuspidatum to armour oxygen estwood drug resistant staphylococcus aureus,multiple drug resistant pseudomonas aeruginosa,and ESBLs escherichia coli[J]. Laboratory Medicine & Clinic, 2012.
  3. Velikova, N.; et al. Putative histidine kinase inhibitors with antibacterial effect against multi-drug resistant clinical isolates identified by in vitro and in silico screens[J]. Rep, 2016, 6:26085.
Online Inquiry
Please note: Our products and services are not intended to be used directly in diagnostic or therapeutic procedures.
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